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Institute for Chemical Imaging of Living Systems

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Welcome to Northeastern University FBS Portal. This site is designed to automate the use of our Core Facilities and to provide the best possible customer service.

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Our Core Facilities

To learn more about a particular facility or to request access, please click on a facility name below.

Northeastern University

Main Contact Info

Guoxin Rong

805 Columbus Ave, ISEC 080
Boston, MA 02115

cils-core@northeastern.edu

(617) 373-4565

Remittance Contact Info

Guoxin Rong

360 Huntington Ave
Guoxin Rong - ISEC 129
Boston, MA 02115

cils-core@northeastern.edu

(617) 373-4565

This facility has not published any Products. Please check back.

The following Products and Services are available within our facility:

Biophysical Core

	NanoTemper Monolith NT.Automated Fast measurement: Kd in about 10 min Low sample volume Wide Kd range from nM to mM range Immobilization free, in-solution measurements Measurements in complex mixtures
	Bio-rad ChemiDoc MP Gel Imager Located in MU 220D Biology Department Facility; $50/quarter/lab Imaging gels and blots UV Tray: EtBr, RGB/far red/NIR fluorescence, SYBR stain White Tray: Coomassie blue, copper, silver, zinc stains Blue Tray: GelGreen, GelStar, Fluorescein

Flow Core

	Beckman Coulter CytoFLEX S 4 laser lines (405, 488, 561, 638). 13 color detection Can load tube or various well plates for analysis Violet side scatter for small particle detection Able to interface experiment design into SRT
	Beckman Coulter CytoFLEX SRT 4 laser lines (405, 488, 561, 638). 15 color detection 100nm nozzle Sort into 1-4 tubes, slides, or into various well plates Mixed Mode sorting Violet side scatter for small particle detection

Microscopy Core

	ZEISS LSM 800 Laser line: 405, 488, 561, 640 nm Objective: 5x, 10x, 20x, 40x, 63x Fixed cell/tissue imaging, Image Tiling
	ZEISS LSM 880 Laser line: 405, 458, 488, 514, 561, 594, 640 nm Objective: 10×, 20×, 40×, 40×, 63× Cage incubator, CO2/O2 control, Superresolution (Airyscan) Spectral Unmixing
	ZEISS LSM 880 NLO Laser line: 405, 458, 488, 514, 561, 594, 640 nm NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm Objective: 10×, 20×, 20×, 40×, 20×-CLARITY Multi-photon imaging Superresolution (Airyscan) Spectral Unmixing
	ZEISS LSM 900/BRUKER NANOWIZARD 4XP AFM: DirectOverlay integration with optical microscopy Large scan field of 100 × 100 × 15 µm Atomic lattice resolution Confocal: Laser line: 405, 488, 561, 640 nm Objective: 10×, 20×, 63× Live cell imaging with environmental control
	ZEISS LIGHTSHEET Z1 Laser line: 405, 488, 561, 638 nm Objective: 5×, 10×-water, 20×-CLARITY CO2/temperature control, Large scale 3D tissue imaging arivis Vision4D software
	Zeiss AXIOZOOM V16 Continuous zoom with high NA Large filed of view Apotome.2 Structured Illumination Module In vivo and model organism Imaging
	ZEISS AXIO OBSERVER (DEMO) Epifluorescence: DAPI, GFP, DsRed, Cy5 Objective: 2.5x, 10x, 20x, 40x, 63x immunofluorescence-labeled tissue or cell culture imaging

Preclinical Imaging Core

	REVVITY IVIS SPECTRUM in vivo and in vitro bioluminescence and fluorescence imaging High throughput: 5 mice with 23cm FOV Excitation: 415-760nm; Emission: 490-850 nm spectral unmixing 3D bioluminescence/fluorescence tomography 3D co-registration with Quantum GX3 microCT
	REVVITY QUANTUM GX3 X-ray: 20-100 kV, 200 uA, 20W; Field of view: 8-86 mm Resolution: 10 um for 15 min scan of 8mm sample; max resolution: 5um Respiratory and cardiac gating Co-registration with IVIS Spectrum
	BRUKER BIOSPEC 3T MRI Automated animal positioning Multimodal rat/mouse cradle Two body volume coils for rat/mouse SAII 1030 monitoring/gating module Localized MR Spectroscopy
	VISUALSONICS ULTRASOUND VEVO F2 Linear probe: 57-25 MHz For high-resolution mouse cardiovascular, abdominal, reproductive; embryology; tumor imaging (< 14 mm diameter); small rat vascular Linear probe: 29-15 MHz For rat cardiac & abdominal (<400 g); large tumor imaging (<23 mm diameter); all contrast applications Phased probe: 8-4 MHz For large animal cardiology & abdominal
	PRECISION XRAD 320 Programmable irradiation on cells and animals 320kV, 12.5mA, 4000W 2.5Gy/min at 50cm SSD with beam hardening filter Adjustable collimators and specimen shelf OptiMAX module for 2D X-ray imaging Bioluminescence, fluoroscopy, radioisotopic imaging Real-time specimen viewing
	PHOTON ETC IR VIVO Illuminaton: Laser at 670, 760, 808 nm Emission Range: 900-1600 nm Lens: 50mm f/1.4 FOV: 5 x 4 to 15.6 x 12.5 cm Heated Stage

Please acknowledge CILS Core in your publication! We suggest the following text in the acknowledgements section of your paper: “We thank the Institute for Chemical Imaging of Living Systems at Northeastern University for consultation and imaging support”

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Upon submitting your request, you will be sent a verification email message. Once verified, your contact information will be forwarded to the facility. If you have any questions, please contact the facility directly: Guoxin Rong cils-core@northeastern.edu Note: Fields denoted with an asterisk ( * ) are required.

Northeastern University

Main Contact Info

Guoxin Rong

Remittance Contact Info

Guoxin Rong

Biophysical Core

NanoTemper Monolith NT.Automated

Bio-rad ChemiDoc MP Gel Imager

Flow Core

Beckman Coulter CytoFLEX S

Beckman Coulter CytoFLEX SRT

Microscopy Core

ZEISS LSM 800

ZEISS LSM 880

ZEISS LSM 880 NLO

ZEISS LSM 900/BRUKER NANOWIZARD 4XP

ZEISS LIGHTSHEET Z1

Zeiss AXIOZOOM V16

ZEISS AXIO OBSERVER (DEMO)

Preclinical Imaging Core

REVVITY IVIS SPECTRUM

REVVITY QUANTUM GX3

BRUKER BIOSPEC 3T MRI

VISUALSONICS ULTRASOUND VEVO F2

PRECISION XRAD 320

PHOTON ETC IR VIVO